University of Otago researchers have helped characterise a genetic variant that enables new understanding of why some
people are at risk of gout, a painful and debilitating arthritic disease.
Gout is caused by persistently elevated levels of urate in the blood, which causes severe joint pain and swelling,
especially in peoples’ toes, knees, elbows, wrists and fingers. It can be treated using drugs that lower urate levels.
But if left untreated, it can cause serious damage to joints, kidneys and quality of life.
PhD student Sarada Ketharnathan together with Associate Professor Julia Horsfield from the Department of Pathology and
Professor Tony Merriman from the Department of Biochemistry have recently characterised the genetic variant that lies
not inside, but just next to a gene called PDZK1.
This PDZK1 protein product helps excrete urate through the kidney and gut. In this way, PDZK1 controls the amount of
serum urate which, when high, form crystals that cause gout.
“We found that the genetic variant doesn’t affect the PDZK1 protein, but causes change in the amount of the PDZK1 gene
produced,” Associate Professor Horsfield explains.
“Unexpectedly, the effect of the genetic variant in humans is in the gut as well as the kidney. We confirmed this by
studying where the variant switches on gene expression in zebrafish embryos, which are ideal because their embryos are
transparent,” she says.
“Our results have identified a new molecular pathway for gout, enabling new understanding of why there is gout risk in
patients with this particular genetic variant.”
The research, which also involved researchers from the University of Auckland, was recently published in the journal
Human Molecular Genetics. It was funded by the Health Research Council of New Zealand.
Associate Professor Horsfield explains there are dozens of regions in the human genome with signals that increase serum
urate levels and risk of gout, and kidney function. The new scientific challenge is converting these signals into
functional insights. This information is critical to allow new medical intervention in gout and kidney disease.
“Since many of the regions lie outside of genes, it is not known how they could control urate levels and risk of gout or
kidney disease. These associated regions probably represent regulatory elements that control gene expression,” she says.
Understanding how genetic variation contributes to someone’s risk of gout or kidney disease can, going forward, inform
choice of treatment for that person.
“This kind of scientific understanding of disease risk is bringing us into a new age of ‘precision medicine’.”